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KMID : 1007520230320121617
Food Science and Biotechnology
2023 Volume.32 No. 12 p.1617 ~ p.1629
An overview of various methods for in vitro biofilm formation: a review
Han A-Reum

Lee Sun-Young
Abstract
Biofilms are widely present in the natural environment and are difficult to remove as they are a survival strategy of microorganisms. Thus, the importance of studying biofilms is being increasingly recognized in food, medical, dental, and water quality-related industries. While research on biofilm detection methods is actively progressing, research on biofilm formation is not progressing rapidly. Moreover, there are few standardized methods because biofilm formation is affected by various factors. However, comprehensive knowledge of biofilm formation is essential to select a suitable method for research purposes. To better understand the various in vitro biofilm formation methods, the principles and characteristics of each method are explained in this review by dividing the methods into static and dynamic systems. In addition, the applications of biofilm research based on various assays are also discussed.
KEYWORD
In vitro biofilm formation, Static method, Dynamic method, Biofilm devices, Biofilm analysis
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